Absorbable coral hydroxyapatite as a scaffold for tissue engineered bone in vitro
- VernacularTitle:Interpore 500R复合人骨髓基质干细胞构建组织工程骨
- Author:
Gang CAO
;
Tianoiu MAO
;
Qing XI
- Publication Type:Journal Article
- Keywords:
Bone;
Marrow stromal stem cells;
Absorbable;
Hydroxyapatite;
Collagen
- From:
Journal of Practical Stomatology
1996;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
?Objective:To investigate the behavior of absorbable coral hydroxyapatite (Interpore 500R) as bone tissue engineering scaffold. Methods: Marrow stromal stem cells harvested from legally aborted human fetus were cultured in DMEM containing 100 ml/L fetal boving serum and induced to differentiate towards osteoblasts.Then the cells were seeded onto absorbable coral hydroxyapatite , then the composite was implanted subcultaneously into the back of nude mice .Cell materia attachement was observed with scanning electron microscope,type I collegen secretion was studied with laser confocal microscope and new bone formation was studied histologicaley. Results: The cells could adhere to absorbable coral hydroxyapatite and secrete collagen I.Mature bone tissue was found in the implanted composite 40 day after transplantation. Conclusion: Absorbable coral hydroxyapatite Interpore 500R is a favorable bone tissue engineering scaffold.
