Constructs and expression of hepatitis C virus core gene combined hepatitis B virus core gene with two multiple cloning sites vector
- VernacularTitle:丙肝病毒(HCV)及乙肝病毒(HBV)双表达载体的构建及表达
- Author:
Tao DENG
;
Gongren FAN
;
Tianbao CHEN
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus Hepatitis C virus DNA-based immunization
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a HCV combined HBV DNA-based therapeutic vaccine.Methods:The HBV core gene and HCV core cDNA were inserted into the eukaryotic expression vector with two multiple cloning sites mammalian expression vector under the CMV promoter and RSC promoter respectively, named pRSC-HBV/HCV. Cellular expression of pRSC-HBV/HCV was assessed by Western blot and immunofluorescent study following transfection into SP2/0 cells. The Balb/c mice were immunized by multiple sites intramuscular injection with pRSC-HBV/HCV and the immune responses were detected.Results:The 21 kD and 14 kD core protein were observed. Both anti-HBc Ab and anti-HCV core Ab were detected in all immunized mice.Conclusion:The investigation demonstrated that pRSC-HBV/HCV could express HBcAg and HCV core protein, humoral immune response could be detected in mice immunized with pRSC-HBV/HCV.
