Expression of hepatitis B virus DNA vaccine in immunized mice
- VernacularTitle:乙肝表面抗原核酸疫苗在小鼠体内的阳性表达
- Author:
Jianping WEN
;
Guizhen YANG
- Publication Type:Journal Article
- Keywords:
Hepatitis B DNA vaccine Expression
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To research the expression of HBsAg DNA vaccine in immunized mice.Methods:The expression of HBsAg DNA vaccine was detected by immunohistochemistry staining in muscle fibers,lymphoid nodes and spleens of mice after intramuscular injection.Anti-HBsAg antibody was assayed by ELISA and the proliferation of spleen cells was measured by MTT.Results:The positive expression was observed in the tissues mentioned above.The titer of serum antibody showed positive results.Spleen cells could be proliferated by stimulating with HBsAg or ConA.Conclusion:The positive expression and certain kinds of immune responses have been shown in immunized mice.
