Studies on the immunotherapy of EAE by adoptive transfer of immunoregulatory T cells specific for the spreading determinant
- VernacularTitle:扩散性抗原决定簇特异性Th2/Tr1细胞被动免疫治疗实验性变态反应性脑脊髓炎的研究
- Author:
Ling YIN
;
Chunjie SONG
;
Ke ZHU
- Publication Type:Journal Article
- Keywords:
EAE T lymphocyte Tr1 cell Epitope spreading Immunotherapy
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the therapeutic potential of preemptive targeting of the epitope spreading cascade by adoptive transfer after EAE onset of immunoregulatory T cells specific for the MBP87-99 spreading determinant.Methods:EAE was induced by PLP139-151 in SWXJ mice, all mice were weighed and examined daily for neurologic signs. On days 6 after EAE onset, mice were injected with IL-2prom→IL-10cDNA-transfected Th2/Tr1-like T cells specific for MBP87-99,PLP104-117,or BSA. Finally demyelination was quantified by analysis of digitized images of PLP immunostained spinal cord sections.Results:Mice received Th2/Tr1-like T cells specific for MBP87-99 had a significantly improved clinical outcome(P=0.02) compared with control mice, reduction in the relapse rate and delay in mean time to onset of first relapse were also observed(P
