Comparative study of EF and kidney-tonifying recipes on regulatory effect of T lymphocytes apoptosis genes in Corticosterone-treated rats
- VernacularTitle:淫羊藿总黄酮与补肾复方对皮质酮大鼠T细胞凋亡相关基因群调控的对比研究
- Author:
Ziyin SHEN
;
Yu CHEN
- Publication Type:Journal Article
- Keywords:
Corticosterone-treated rats EF Kidney-tonifying recipes Spleen-invigorating recipe T lymphocytes apoptosis Genes regulation
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the regulatory effect of EF and two Kidney-tonifying recipes as well as Spleen-invigorating recipe on expression of T lymphocytes apoptosis related genes in Corticosterone-treated rats.Methods:To quantify and compare the T lymphocytes apoptosis percentage and the expression the pro-apoptotic and anti-apoptotic genes related to T cell apoptosis in every experimental groups using TUNEL method and flow cytometry assay, as well as the fluorescence real time PCR technology.Results:The percentage of T cell apoptosis and the expression of apoptosis related genes were significantly regulated by EF and two Kidney-tonifying recipes, while the Spleen-invigorating recipe had no significant effect with respect to these parameters.Conclusion:EF can be used to represent the effect of Kidney-tonifying recipes in antagonizing the inhibiting effect of exo-corticosterone with respect to T lymphocytes.
