Study on the Oxidative Damage Effects in Rat Lung Type Ⅱ Cells Exposed to Cooking Oil Fume
- VernacularTitle:食用油烟对大鼠肺Ⅱ型细胞氧化损伤作用的研究
- Author:
Geyu LIANG
;
Hui YUAN
;
Lihong YIN
- Publication Type:Journal Article
- Keywords:
Plant oils;
Cookery;
Oxidative damage;
N_acetylcysteine
- From:
Journal of Environment and Health
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study possible mechanisms of oxidative damages in rat lung type Ⅱ cells induced by cooking oil fume (COF). Methods The rat type Ⅱ lung cells were pretreated with anti_oxidant N_acetylcysteine (NAC) for 30 miuntes and were exposed to COF for 12 hours. The contents of MDA and GSH were detected with thiobarbituric acid colorimetric assay and 5,5_dithiobis 2_nitrobenzoic acid colorimetric assay. Results The significant increase of the contents of MDA and significant decrease of the contents of GSH were observed in the rat lung type Ⅱ cells with the increase of exposure doses of COF and the prolongation of exposure time of COF. The pretratment of NAC could reduce the production of MDA and increase the contents of GSH of the cells. Conclusion The possible mechanisms of oxidative damages in rat lung type Ⅱ cells induced by cooking oil rume might be formation of lipid peroxidation and interference of GSH anti_oxidative systems of the cells.
