Studies on Un-fluorescent Staining Methods in Comet Assay
- VernacularTitle:彗星试验非荧光染色研究
- Author:
Rong ZHAO
;
Zhengchang HENG
- Publication Type:Journal Article
- Keywords:
DNA damage;
Laboratory diagnosis;
Comet assay;
Silver stain method
- From:
Journal of Environment and Health
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an un_fluorescent staining method in Comet Assay. Methods Some special un_fluorescent DNA staining methods, Feulgen, Methyl Green, Stains_all and Silver_Stain were tested in Comet Assay and the comparison studies with EB stain were carried out. Results Among the stain methods tested, just the silver_stain could give a satisfactory effect: legible, ease to judge the stained area of the comets head and tail, permanent, sensitive, could make little segment of DNA be stained, which couldnt be stained by EB stain, the length of comets head and tail, were over 2 times of that of EB stain, safety and economical. Conclusion The silver_stain method discarded the defects of EB stain, so that it could replace EB stain and make comet assay possible to be extended.
