The affection of recombinant adenovirus vector expressing wild type p53 to prostate cancer cell line PC3M
- VernacularTitle:腺病毒介导的野生型p53对前列腺癌细胞系PC3M的影响
- Author:
Ming LIU
;
Jianye WANG
;
Jian LI
- Publication Type:Journal Article
- Keywords:
Prostate neoplasms;
Carcinoma;
P53 gene
- From:
Chinese Journal of Urology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the availability and mechanism of recombinant adenovirus vector expressing wild type p53 in gene therapy of prostate cancer. Methods Prostate cancer cell line PC3M was cultivated and recombinant adenovirus vector expressing wild type p53 was added to the media. Then the levels of proliferation and apoptosis were detected and changes of bax were exa mined . Results AdCMVp53 is effectively transfected to the cells. The transfecting ratio is 95%. In the cells transfected with AdCMVp53, the level of proliferation decreased. Tested by BrdU, the absorbance rate decreased from 1.454 in the control group to 0.496 in the experimental group( P
