The Effects of Vitamin C on the Growth and Apoptosis of Hela Cell
- VernacularTitle:维生素C对宫颈癌HeLa细胞增生和细胞凋亡的影响
- Author:
Yanhua WANG
;
Liming HUANG
- Publication Type:Journal Article
- Keywords:
Cervix neoplasms;
Vitamins;
Papillomavirus
- From:
Journal of Medical Research
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the effects of vitamin C in vitro,on human cancer HeLa cell multiplication and apoptosis, in order to promote vitamin C in the tumor treatment application. Methods the morphology changeof cervical cancer Hela cell was observed under the inverted microscope;the effects of vitamin e on Hela cell by MTT assay; cell cyele and weakly perishing rate was analysed rate was analysed by Flow CytoMeter;applying the agarose gelatin electricity swims to analyse weakly perishes in the process the cell gene group DNA change. Results Vitamin C hinders the HeLa cell by the density and the time dependence way to the G0/G1 period, suppresses it to grow and to induce weakly perishes. Conclusions Vitamin C may suppress the HeLa cell to grow, causes the cell to hinder to the G0/G1 period and induces cell weakly perishing.Its reason possibly was HPV E6 protein expression surrenders to restoring the function of P53 gene.
