Associaton of polymorphism of endothelial nitric oxide synthase gene with essential hypertension and type 2 diabetes mellitus
- VernacularTitle:内皮型一氧化氮合酶基因多态性与原发性高血压、2型糖尿病的关系
- Author:
Huibin HUANG
;
Lixiang LIN
;
Mingqin CHEN
- Publication Type:Journal Article
- Keywords:
Hypertension;
Diabetes mellitus, non-insulin-dependent;
Polymorphism (Genetics);
Nitric-oxide synthase;
Genes
- From:
Chinese Journal of Endocrinology and Metabolism
1985;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association of the polymorphism of endothelial nitric oxide synthase (eNOS) gene 27bp variable number of tandem repeats (VNTR) with essential hypertension (EH) and type 2 diabetes mellitus (DM) in Chinese population. Methods (1) Genotypes were determined by polymerase chain reaction (PCR)-agarose gel electrophoresis. (2) Fasting serum nitric oxide metabolites (NOx) were measured by nitrate reductase. (3) During oral glucose tolerance test (OGTT) serum immunoreactive insulin (IRI) and C peptide (CP) were measured by radioimmunoassay. Results (1) The frequenciesofaalleleinEHandtype2DMgroupwere significantly higher than that in control group (0.109 vs 0.051; 0.129vs 0.051, P
