Long-term behavioral recovery in Parkinsonian rats by triple transduction with dopamine-biosynthesizing enzyme genes
- VernacularTitle:帕金森病大鼠三重目的基因共表达的长期行为改善
- Author:
Yang SHEN
;
Dongsheng FAN
;
Lijun WANG
- Publication Type:Journal Article
- Keywords:
Dependovirus;
GTP cyclohydrolase;
Parkinson disease;
Gene therapy
- From:
Chinese Journal of Neurology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of gene therapy for Parkinson disease by triple transduction with dopamine-biosynthesizing enzyme genes. Methods We constructed high titer purified adeno-associated virus (AAV) vectors containing cDNAs encoding human tyrosine hydroxylase (TH), aromatic L-amino acid decarboxylase (AADC) and GTP cyclohydrolase I (GCH) respectively. AAV-TH, AAV-AADC and AAV-GCH were stereotaxically injected into the denervated striatum of Parkinsonian rat; the expression of TH, AADC or GCH was assayed by using immunohistochemical staining; the rotational behavior was assayed for 1 year.Results The immunohistochemical staining and behavioral testing showed that all of TH, AADC and GCH were expressed stably in the striatum, and the rats treated with AAV-TH, AAV-AADC and AAV-GCH showed more remarkable decrease in rotation rate persisted for at least 1 year, than those treated with AAV-TH and AAV-AADC(P
