A cDNA microarray study on differentially expressed genes in prostate cancer
- VernacularTitle:应用基因微矩阵芯片筛选前列腺癌的相关基因
- Author:
Liqun ZHOU
;
Xuezhen YANG
;
Xiao HUANG
- Publication Type:Journal Article
- Keywords:
Prostatic neoplasms;
Carcinoma;
Genes;
DNA chip
- From:
Chinese Journal of Urology
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect differentially expressed genes in prostate cancer by cDNA microarray. Methods Total RNA was isolated by one step protocol from prostate cancer (CaP) and from normal prostate,and Poly(A) +RNA was further purified through Oligitex mRNA Midi Kit.Then it was analysed for differentially expressed genes in CaP and normal prostate by cDNA microarray with 4 096 human genes. Results There were 341 differentially expressed genes between CaP and normal prostate,of which there were 128 down regulated and 213 up regulated ones for CaP. Among these genes,15 were the most significant with 6 down regulated and 9 up regulated. Conclusions cDNA microarray can be used effectively to find out differentially expressed genes in prostate cancer which is not regulated by any single gene.Many different kinds of genes are involved in the initiation and evolution of CaP carcinogenisis.
