Changes of DNA content in the fibroblasts of narrow pedicled subdermal vascular network flap during its surviving
- VernacularTitle:窄蒂真皮下血管网皮瓣成纤维细胞DNA含量的变化
- Author:
Bin ZHU
;
Jianhua GAO
- Publication Type:Journal Article
- Keywords:
Subdermal vascular network flap;
Narrow pedicle;
Fibroblast;
DNA
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2002;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the dynamic changes of DNA content of the fibroblasts in narrow pedicle subdermal vascular network flap (SVNP) during their surviving. Methods Eighteen pieces of surviving skin in pig back with size of 16 cm?8 cm and pedicle wideness of 4 cm were stained with acridine orange, and their DNA contents were measured with the adherent cell analysis and sorting cytometer 570. The tissue samples were taken at 3, 4 ,5 ,and 7 days after operation from different parts of the flap.Results The DNA content of fibroblast in the proximal part of the flap was slightly changed, those in the middle portion increased from 3rd to 5th day and became normal at 7th day, and in the distal portion progressively increased after operation. The results suggested that the transitional part of the flap and skin graft in the middle portion was most active in function and metabolism, and well survived, but the distal portion of the graft was poor in surviving and had a long repairing period. These indicated that local tissue hypoxia stimulated proliferation of fibroblasts and synthesis of the DNA in a short time, but as time wert by, the proliferative speed and DNA synthesis in the individual cell were heterogeneous, probably because of the degeneration or necrosis of some epidermal cells in the graft. Conclusion The wideness and length of the pedicle as well as its vascular anastomoses should be readjusted to promote the blood circulation of the distal skin graft tissues.
