Effects of calcitonin gene-related peptide on calcification of human dental pulp cells in vitro
- VernacularTitle:降钙素基因相关肽对人牙髓细胞的矿化影响
- Author:
Qun LU
;
Buling WU
;
Hongwei ZHANG
- Publication Type:Journal Article
- Keywords:
Calcitonin gene related peptide;
Dental pulp;
Alkaline phosphatase;
Collagen;
Osteocalin;
Cells
- From:
Journal of Practical Stomatology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
砄bjective: To investigate the effect of calcitonin gene related peptide(CGRP) on calcification function of human dental pulp cells(HDPCs) in vitro . Methods: HDPCs cells were exposed to calcitonin gene related peptide at the dosages(mol/L) of 10 -12 , 10 -10 and 10 -8 for 3,6,9, 12 and 15 days respectively. Cell growth was studied by cell counting , alkaline phosphatase(AL) by a AL kit, osteocalin by immunoradiological tecnique and type I collagen mRNA expression by RT PCR. Results: CGRP did not show any effect on the growth of HDPCs, but it increased the expression of AL, osteocalin and type I collagen mRNA in HDPCs in a dose dependent and not time dependent manner. Conclusion: CGRP plays an important role in promoting differentiation and calcification function of HDPCs
