Application of thiocyanate elution in the selection of phage antibodies with high affinity
- VernacularTitle:用硫氰酸盐洗脱法筛选高亲和力噬菌体抗体
- Author:
Gang WANG
;
Yan WANG
- Publication Type:Journal Article
- Keywords:
Thiocyanate elution Phage antibody Antibody affinity
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the selection of phage antibodies(Phabs) with higher affinity.Methods:Simulant libraries were constructed using anti keratin and anti HBsAg Phabs with different affinities.During the panning procedure,thiocanate solutions of different concentrations were used as elution buffers to favor the recovery of Phabs with higher affinities.The recovered Phabs were identified by restriction enzyme mapping.Results:All the tested Phabs were proved tolerant to thiocyanate treatment.By panning the simulant libraries,the ratio of Phabs with higher affinity in the obtained clones were increased alonging with the increase of NH 4SCN concentrations.Conclusion:Thiocyanate elution is a powerful and effective method to help obtain the Phabs with high affinity from phage antibody library.
