The enhancing effect of human interleukin-18 expressed in nasopharyngeal carcino-ma cells on the cytotoxicity of CD8+ T cells from patient with nasopharyngeal carci-noma
- VernacularTitle:鼻咽癌细胞中表达的人白细胞介素18(hIL-18)对鼻咽癌患者外周血CD8~+T细胞细胞毒活性增强作用的研究
- Author:
Jianping TAO
;
Yunfei XIA
;
Qing ZHANG
- Publication Type:Journal Article
- Keywords:
Interleukin-18 Expression CD8+T cell Cytotoxicity Perforin Nasopharyngeal carcinoma
- From:
Chinese Journal of Immunology
1985;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The study is designed to investigate if human interleukin 18 (hIL-18) cDNA expressed in nasopharyngeal carcinoma (NPC) cells could enhance the cytotoxicity of CD8+ T cells from the PBMC of the MFC-bearing patient and how to enhance it.Methods: A constructed eukaryotic expression vector for human IL-18 ,pcDNA3.1(-)/hIL-18, was used to transfect the NPC cell strain-SUNE cells. Then, the transfected SUNE cells were mix-cultured with CD8+ T cells which were separated from the PBMC of the patient with NPC, the cytotoxicity was measured by lactate dehydrogenase(LDH) releasing method, the expression for Perforin, Fas-L and Granzyme B were detected with the mix-cultures. Results: The eukaryotic expression vector could highly express hIL-18 in transfected SUNE cells and only traces of the protein exist in non-transfected SUNE cells. The concentration of hIL-18 in supernatant of the transfected SUNE cells was (85 ? 10) pg/ml, but less than 5 pg/ml of hIL-18 contained in supernatant of the non-transfected SUNE cells.The expression of hIL-18 in SUNE cells could enhance the cytotoxicity of CD8+ T cells from the NPC patient significantly( P
