The Morphometric Changes of the Enteric Nervous System in the Intestinal Transection-reanastomosis Model.
10.11637/kjpa.2007.20.1.21
- Author:
Soo Youn OH
1
;
Ung Chae PARK
;
Kyung Rae KIM
;
Jun Ho WANG
;
Jae Dong LEE
;
Soon Je KIM
;
Wu Chul SONG
;
Seung Hwa PARK
Author Information
1. Department of General Surgery, College of Medicine, Konkuk University, Korea.
- Publication Type:Original Article
- Keywords:
Intestinal transection-reanastomosis;
Myenteric plexus;
Type 1 interstitial cells of Cajal (ICC-Is);
Regeneration;
Morphometry
- MeSH:
Animals;
Cell Count;
Cytoplasm;
Enteric Nervous System*;
Ether;
Interstitial Cells of Cajal;
Intestine, Small;
Laparotomy;
Myenteric Plexus;
Neurons;
Rats;
Rats, Sprague-Dawley;
Regeneration;
Silk;
Sutures;
Vacuoles
- From:Korean Journal of Physical Anthropology
2007;20(1):21-34
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was performed to investigate the morphometric changes of myenteric plexus and type 1 interstitial cells of Cajal (ICC-I) in regeneration process of small intestine transection. Sprague Dawley rats (200~250 g) were anesthetized with ether; then the full thickness of ileal wall were semitransected; and subsequent end-to-end anastomosis were performed by using 6-0 silk suture thread. Sham-operated rats, which only underwent the laparotomy, were used for control group. Experimental animals were sacrificed at 3 days, 7 days, 15 days, and 30 days after the operation. In each group myenteric plexus and ICC-Is were prepared by histochemical method (NADH-TR stain for myenteric plexus, ZIO stain for ICC-Is) and cell numbers were counted by image analyzer (Image plus pro-5.0, Media Cybermedics, USA). Degeneration of myenteric neurons and ICC-Is occurred simultaneously and it was similar in oral and anal to the site of transection. Degeneration effects were most prominent at 3 days and 7 days after intestinal transection. In myenteric plexus, many neurons had degenerated appearances and about 40% of them were lost. Most of ICC-Is had cytoplasmic vacuoles and 20~37% of the cells were lost. At 15 days after transection, there were no more degeneration in myenteric neurons (20~25% cell loss) and ICC-Is (20~13% cell loss). At 30 days after transection, numbers of myenteric neurons were not recovered as that of the control group. However numbers of ICC-Is were as similar as that of the control group. In conclusion, we confirmed that degeneration effects of intestinal transection are more severe in myenteric plexus than in ICC-Is, and recovery of cell loss occurs more slowly in myenteric plexus.
