Association of single nucleotide polymorphisms of glucagon gene and essential hypertension in Shanghai Chinese
- VernacularTitle:上海汉族人胰高血糖素基因单核苷酸多态性与原发性高血压的相关性
- Author:
Kuixing ZHANG
;
Ying WANG
;
Dingliang ZHU
- Publication Type:Journal Article
- Keywords:
Glucagon;
Gene;
Hypertension;
Polymorphism (Genetics);
Single nucleotide polymorphism
- From:
Chinese Journal of Endocrinology and Metabolism
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect and genotype the single-nucleotide polymorphisms (SNPs) in coding and promoter regions of human glucagon gene in Han Chinese residing in Shanghai and to analyse its association with essential hypertension (EH). Methods The identification of SNPs was performed by both direct DNA sequencing and denaturing high-performance liquid chromatography (DHPLC). For genotyping of SNPs direct sequencing was performed in 96 patients with EH and 96 normotensive controls (NT). Results Two SNPs in glucagon gene, one in the coding region (C3689T) and the other in the joint region, (G5505A) were found. A higher frequency SNP, C3689T, was genotyped and no significant difference in C3689T genotype frequency was found between EH and NT. Conclusion There is an important ethnic difference in SNP distribution of human glucagon gene. The distribution of C3689T genotype in Han Chinese is not different between EH and NT.
