Study on TGF-?1 mRNA transcription of peripheral blood mononuclear cell in type 2 diabetic patients with nephropathy
- VernacularTitle:2型糖尿病肾病患者外周血单个核细胞TGF-?1 mRNA转录的定量研究
- Author:
Wuqiang FAN
;
Hong LI
;
Weimin CAI
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathies;
Transforming growth factor beta;
Reverse transcriptase polymerase chain reaction;
Dot blotting
- From:
Chinese Journal of Endocrinology and Metabolism
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clinical significance of quantitation of TGF-?1 mRNA of peripheral blood mononuclear cell (PBMC) in type 2 diabetic patients at different stages of nephropathy (DN). Methods TGF-?1 mRNA transcription in PBMC was analyzed quantitatively by RT-PCR and dot blot in 93 cases of type 2 diabetic patients and 35 normal controls. Correlation of TGF-?1 mRNA in PBMC with 24 hours urinary albumin excretion rate (UAER) was also analysed. Results TGF-?1 mRNA level of PBMC in type 2 diabetic patients was significantly higher than that in normal controls (1.56?1.00 vs 1.03?0.25, P
