Effect of iodine on apoptosis in cultured human thyrocytes
- VernacularTitle:碘对人甲状腺细胞凋亡的影响
- Author:
Chao LIU
;
Xiaohong WU
;
Youwen QIN
- Publication Type:Journal Article
- Keywords:
Iodine;
Thyroid gland;
Apoptosis;
Fas;
Soluble Fas;
Bcl-2;
Bak
- From:
Chinese Journal of Endocrinology and Metabolism
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of iodine on apoptosis of thyrocytes and to study the effect of iodine on the pathogenesis of thyroid diseases. Methods Normal human thyrocytes were cultured in the absence or presence of 10 -8 ～10 -4 mol/L NaI. Apoptosis, Fas expression, Bcl-2 and Bak expression and Fas and soluble Fas (sFas) mRNA levels in thyrocytes were detected by flowcytometry, Western blot and semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) respectively, sFas was detected in supernatant of cultured thyrocytes by ELISA. Results (1) Low concentration of iodine (10 -8 mol/L) could inhibit apoptosis, while high concentrations of iodine (10 -6 ～10 -4 mol/L) increased apoptosis (P
