The effect of glucose transporter 1 on hexosamine biosynthesis pathway in rat glomerular mesangial cells
- VernacularTitle:葡萄糖转运蛋白对大鼠肾小球系膜细胞己糖胺通路的影响
- Author:
Zhihong LIU
;
Yingjian LI
;
Jiaming ZHU
- Publication Type:Journal Article
- Keywords:
Glomerular mesangium;
Mesangial cell;
Glucose transporter 1;
Extracellular matrix;
Hexosamine biosynthesis pathway
- From:
Chinese Journal of Endocrinology and Metabolism
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of hexosamine biosynthesis pathway (HBP) in the functionalalterationofmesangialcellstransinfectedwith glucose transporter 1 (GLUT1) gene. Methods Rat mesangial cells were transinfected with the human GLUT1 gene (MCGT1) by retrovirus vector. Mesangial cells transinfected with bacterial ?-galactosidase (MCLacZ) were used as control. Glucose uptake was detected with 2-deoxy-〔 3H〕-D-glucose (2-DG). Cell size, RNA/DNA ratio, protein/DNA ratio and the synthesis of fibronectin were evaluated by flow cytometry. The activity of glutamine: fructose-6-P aminotransferase (GFAT), which is the key enzyme of HBP, was assayed by spectrophotometry. The expression of GFAT gene was analyzed by RT-PCR. Results MCGT1 demonstrated higher 2-DG uptake than MCLacZ 〔(741.0?60.5)dpm/?g protein vs (92.2?9.0)dpm/?g protein,P
