Study on the expression of prolactin mRNA in thyroid glands of patients with Graves'disease
- VernacularTitle:Graves病患者甲状腺组织内催乳素mRNA的表达
- Author:
Jing LI
;
Ni LAN
;
Weiping TENG
- Publication Type:Journal Article
- Keywords:
Graves'disease Thyroid Prolactin Autoimmunity
- From:
Chinese Journal of Immunology
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Abstract Objective:To investigate whether mRNA of prolactin(PRL) ,an important immunomodulatory hormone,is expressed in thyroidglands of patients with Graves'disease(GD) .Methods:The expression of PRL mRNA was examined by nonisotopic in situ hybridization in GDthyroid glands and the control ones( including multinodular goiters and samples of normal thyroid tissue adjacent to adenomas) as well as intra-thyroidal mononuclear cells isolated from GD thyroid glands and primary thyroid follicular cell(TFC) cultures derived from them.Results:PRL mRNA was expressed in GD thyroid glands, but not in the control ones. It was localized in infiltrating mononuclear cells and vascular endothelial cells adjacent to mononuclear cell infiltrates, but not in TFC .(9.8?.3)% (x ?s) of intrathyroidal mononuclear cells isolated from GD thy-roid were found containing PRL mRNA.It was absent in the primary TFC cultures. Conclusion:PRL mRNA is expressed in GD thyroid tissue. This indicates that PRL-like substance can be locally produced in GD thyroid tissue, which may play an important role in GD pathogenesis by autocrine and paracrine mechanisms.
