Distribution of HLA-A, B alleles in patients with Guillain-Barr? syndrome
- VernacularTitle:吉兰-巴雷综合征患者人类白细胞抗原A、B的基因分型
- Author:
Chunyan LI
;
Li GUO
;
Weiping WANG
- Publication Type:Journal Article
- Keywords:
Guillan-Barr? syndrome;
HLA-A antigens;
HLA-B antigens;
Genotype
- From:
Chinese Journal of Neurology
1999;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relations between the susceptibility to AIDP and AMAN, two forms of Guillain-Barr? syndrome (GBS), and the frequency of HLA-A, B alleles. Methods A case-control research was done in 31 cases of AIDP, 33 cases of AMAN and 132 health individual controls. DNA was extracted from peripheral blood leukocytes by improved fast saltingout. HLA-A, B antigens were typed by DNA-based technology, PCR-sequence specific primers (PCR-SSP) method. In determination of allelic polymorphism by PCR amplification with SSP, oligonucleotide primers are designed to obtain amplification of specific alleles or groups of alleles. Assignment of alleles is based on the presence or absence of PCR amplified product, which may be detected by agarose gel electrophoresis. Results On research of HLA-A, B alleles polymorphism, it showed that HLA-A33 frequency was increased significantly in AIDP patients [22.6% vs 4.5%,corrected probability (Pc)=0.011]; related risk (RR) was 6.1; HLA-B15, B35 frequencies were increased (51.7% vs 20.8%, Pc =0.015; 34.5% vs 6.9%, Pc=0.000 8); RR was 4.1 and 7.1, respectively. Conclusions There are different distribution of HLA-A, B alleles in AIDP and AMAN, two forms of GBS. AIDP susceptibility is associated with HLA-A33, while AMAN is with HLA-B15, B35.
