Expression of adenoviral mediated NT- 3 genes in Schwann cells of sciatic ne rve in the rats
- VernacularTitle:腺病毒介导的NT-3基因在大鼠坐骨神经的表达
- Author:
Jinyu ZHU
;
Yaotian HUANG
;
Qingsheng ZHU
- Publication Type:Journal Article
- Keywords:
Nerve growth factors;
Adenoviruses,human;
Schwann cells;
Gene;
Sciatic nerve
- From:
Chinese Journal of Orthopaedics
1998;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of neurotrophin- 3 (NT- 3) gene in Schwann cells (SCs) of rat sciatic nerve introduced by adenovir al vector in vivo. Methods Recombinant adenoviral vector for NT- 3 was propagat ed in 293 packaging cells and was titered by tissue culture infections dose 50 ( TCID50) method. Ad- NT- 3 was injected intraneurally into the rat sciatic nerv e following axotomy of the sciatic nerve which was repaired after the immediate injury. Immunohistochemical staining was employed to determine the expression of NT- 3 in SCs in rat sciatic nerve, and the expressive intensity difference was measured with LEICA M550 imagine analysis system on the tissue slides. Results Two days after injection of NT- 3 into the rat nerve, positive stain in the SCs were apparent in the vicinity of anastomosis. There was significant increase of the amount of NT- 3 expression 7 days following injection of Ad- NT- 3 (vs. 2 days group P0.05). Compared with the 2 days group, the 14 and 28 days groups still maintained a relatively high leve l of NT- 3 (P
