TGF-?_1 regulates the expression of multiple genes in prostatic stromal cells in vitro
- VernacularTitle:转化生长因子-?_1对前列腺基质细胞基因表达的调节作用
- Author:
Yuanjie NIU
;
Tengxiang MA
;
Ju ZHANG
- Publication Type:Journal Article
- Keywords:
Prostatic hypertrophy;
Transforming growth factor ? 1;
Genes
- From:
Chinese Journal of Urology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the regulatory effect of TGF ? 1 on the expression of multiple genes in prostatic stromal cells in vitro. Methods The primary culture of prostatic stromal cells (including fibroblasts and smooth muscle cells ) have been established and cultured to 4～6 passages. Then the cells were cultured in the medium with various concentration of TGF ? 1(0.01, 0.10, 1.00 and 10.0 ng/ml)for 48h. By semi quantitative RT PCR method, the androgen receptor(AR), TGF ? 1, bFGF and smoothlin mRNA were measured. Results The expression of AR could be stimulated by low concentration of TGF ? 1 ( P
