Mutations of androgen receptor gene in prostate cancer tissues
- VernacularTitle:前列腺癌组织雄激素受体基因突变的检测
- Author:
Xiaoling YU
;
Jian LU
;
Xiaohui WANG
- Publication Type:Journal Article
- Keywords:
Prostatic neoplasms;
Carcinoma;
Genes;
Receptors,androgen
- From:
Chinese Journal of Urology
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between the mutation of androgen receptor(AR) gene and the development of human prostat e cancer. Methods All exons of AR gene except trineucl eic acid repeat polymorphism were amplified by PCR and selected by single strand conformation polymorphism( SSCP) in 27 paraffin-embedded slides of human pros tate cancer. The abnormal mobility shifts were further detected by direct DNA c ycle sequencing. Results Three abnormal mobility shifts respectively in exon A.D.G were found by SSCP in three prostate cance r tissues. One point mutation was found in 296 amino acid (C 966→A,Ser29 6Arg) from exon A.This point mutation has not been reported before. Conclusions This mutation might change the transcriptive activity of AR, and it might be correlated to PCa progression.
