The impact of polymorphism of calpain-10 gene on glucose intolerance status, insulin secretion and insulin sensitivity
- VernacularTitle:Calpain-10基因多态性与糖耐量异常状态、胰岛素分泌及胰岛素敏感性的相关性
- Author:
Kunsan XIANG
;
Taishan ZHENG
;
Weiping JIA
- Publication Type:Journal Article
- Keywords:
Polymorphism (Genetics);
Diabetes mellitus;
Calpain 10 gene;
NIDDM1 locus;
Insu lin sensitivity
- From:
Chinese Journal of Endocrinology and Metabolism
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the impact of single nucleotide polymorphism 43 (UCSNP43) of calpain 10 gene (CAPN 10) at NIDDM1 locus, chromosome 2q37.3 on glucose intolerance status and its intermediate traits. Methods The studied population consisted of 320 Chinese subjects in Shanghai 〔normal glucose tolerance (NGT) 148, impaired glucose tolerance (IGT) 44 and type 2 diabetes (T2DM) 128〕. Plasma glucose, insulin, C peptide and free fatty acid levels were measured during fasting and 30, 60, 120 and 180 min after oral 75 g glucose load. The tissue insulin sensitivity and islet beta cell insulin secretion were assessed by HOMA formulae and the ratio of the increment of insulin and glucose levels at 30 min. The CAPN 10 UCSNP43 was genotyped by DNA direct sequencing. Results (1) The frequencies of GG, GA and AA genotypes of CAPN 10 UCSNP 43 were 0.80, 0.18 and 0.02 respectively. The G allele frequency was 0.89, which was statistically significant higher than those in Caucasians, Mexican Americans and Pima Indians (all P
