Influence of Aspirin on growth and NOS expression in astrocytoma cell line
- VernacularTitle:阿司匹林对胶质瘤细胞生长增殖及NOS表达的影响
- Author:
Lin XIA
;
Hong DING
;
Fang LIU
- Publication Type:Journal Article
- Keywords:
Aspirin Astrocytoma cell Nitric oxide synthetase Nitric oxide Cancer embryo antigen
- From:
Chinese Journal of Immunology
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Aspirin on the proliferation and NOS expression in astrocytoma cell line,and probe into ins mechanism.Methods:The effect of Aspirin on the growth of astrocytoma cells evaluated by MTT assay;NOS protein levels were determined by immunohistochemistry,NO and CEA concentration in the medium were determined by Griess assay and lepton catch immuning method respectively.Results:Aspirin can inhibit the growth of astrocytoma cells,induce the expression of iNOS,increase the concentration of NO in the medium. The effect of these were in a concentration dependent pattern. Moreover,Aspirin can reduce the concentration of CEA in the medium.Conclusion:Aspirin inhibit the growth of astrocytoma cell line.UP regulated iNOS expression resulting a increase of NO concentration are ascribed to mechanism of antrproliferation activity of Aspirin. CEA is a good indicator in monitoring curative effect of astrocytoma.
