Abnormality of VHL gene and VEGF expression in primary renal cell carcinoma
- VernacularTitle:肾细胞癌VHL基因改变与VEGF表达的关系及意义
- Author:
Fan CHENG
;
Xiaobin ZHANG
;
Jie ZHANG
- Publication Type:Journal Article
- Keywords:
Kidney neoplasms;
Carcinoma;
Genes
- From:
Chinese Journal of Urology
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the significance of t he abnormality of VHL gene and VEGF expression in primary renal cell carcinoma ( RCC). Methods Mutation and abnormal methylation of VHL g ene and the expression of VEGF were dtected by means of multiplex PCR,PCR-SSCP and immunohistochemistry in 42 cases of primary RCC,in 18 specimens adjacent to the neoplastic lesion and in 10 specimens of normal renal tissues. R esults The alteration rate of VHL gene was higher in RCC tissue (61. 9%) than in the normal renal tissue (3.6%,P
