Effects of plasminogen activator inhibitor-1 antisense RNA on aorta endothelial cells cultured
- VernacularTitle:纤溶酶原激活物抑制剂-1反义RNA对主动脉内皮细胞部分功能的影响
- Author:
Lu FU
;
Yu MEI
;
Hui LI
- Publication Type:Journal Article
- Keywords:
Plasminogen inactivators;
RNA, Antisense;
Endothelial growth factor
- From:
Chinese Journal of Geriatrics
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of antisense RNA of plasminogen activator inhibitor 1 (PAI 1) in regulating the expression of PAI 1 and vascular endothelial growth factor (VEGF) in aorta endothelial cells (EC) cultured in vitro. Methods The second extron of PAI 1 was amplified by polymerase chain reaction(PCR) and the product was inserted into eukaryotic cell expression vector pcDNA3.1(-) to construct PAI 1 antisence RNA recombinant plasmid. The recombinant plasmid was transfected into EC and the PAI 1 expression was detected by immunohistochemistry, Westernblot and ELISA. The effects of PAI 1 variation on VEGF were examined by immunofluorescence method. Results PAI 1 antigen was the lowest (0 017 ng/ml) in cells and the immunofluorescence representing the expression of VEGF in the cytoplasm showed the weakest at the third day after transfection. At the fifth day, PAI 1 antigen increased to 0 093 ng/ml with VEGF expression increased correspondingly. At the seventh day, PAI 1 antigen(0 143 ng/ml) and VEGF increased closed to normal level. Conclusions PAI 1 antisense RNA blocked the translation of PAI 1 proteins effectively and inhibited the expression of VEGF in aorta endothelial cells.
