A monoclonal antibody to common acute lymphoblastic leukemia antigen (CALLA) and its expression on several human tumor cell lines.
10.3346/jkms.1992.7.2.147
- Author:
Weon Seo PARK
1
;
Tae Sook HWANG
;
Tae Jin KIM
;
Cheung Seog PARK
;
Young Mee BAE
;
Seong Hoe PARK
;
Sang Kook LEE
Author Information
1. Department of Pathology, Seoul National University College of Medicine, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Common acute lymphoblastic leukemia antigen (CALLA);
monoclonal antibody
- MeSH:
Animals;
Antibodies, Monoclonal/*immunology;
Antigens, Differentiation/*analysis/immunology;
Antigens, Neoplasm/*analysis/immunology;
Flow Cytometry;
Humans;
Immunoglobulin Isotypes/analysis;
Mice;
Mice, Inbred BALB C;
Neoplasms/*immunology;
Neprilysin;
Tumor Cells, Cultured;
Tumor Markers, Biological/*analysis
- From:Journal of Korean Medical Science
1992;7(2):147-153
- CountryRepublic of Korea
- Language:English
-
Abstract:
We describe a newly-made murine monoclonal antibody to the common acute lymphoblastic leukemia antigen (CALLA), named SHB-10. The antigen detected by SHB-10 has a molecular weight of about 105 kDa. This antibody is very similar to that of conventional anti-CD10 Ab on indirect flowcytometric analysis using lymphoid malignant cell lines and peripheral lymphocytes of acute lymphoblastic leukemia (ALL) patients. The binding of anti-CD10 to Daudi cell and peripheral lymphocytes of ALL patients is blocked by SHB-10. Thus this monoclonal antibody is thought to detect the CALLA. The distribution of antigen detected by SHB-10 on several cell lines of neuroectodermal tumor and lymphoid malignancy was analysed and a slight difference in their cell surface expression is observed when compared with that by conventional anti-CD10. Further biochemical analysis is now under way for a better characterization of this antigen.
