DETERMINATION OF THE MOLECULAR WEIGHT OF PHOTOAFFINiTY LABELED GLUCOCORTICOSD RECEPTOR IN SCALDED RAT LIVER CYTOSOL
- VernacularTitle:光亲和标记测定烫伤大鼠肝胞液糖皮质激素受体分子量
- Author:
Yili YANG
- Publication Type:Journal Article
- Keywords:
photoaffinity labeling;
glucocorticoid receptor;
scalding
- From:
Academic Journal of Second Military Medical University
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Previous work in our laboratory showed that, after scalding, there was not only a decrease of rat liver cytosol [3H]-Dexamethasone specific binding sites but also an increase of apparent dissociation constant of [3H]-Dex and a change of sedimentation coefficient of glucocorticoid receptor (GCR).The present study was carried out using photoaffinity labeling of GCR in rat liver cytosol.It was found that there was no significant difference between molecular weights of . GCR in normal and scalded rats determined by SDS-polyacrylamide gel electrophoresis. Our result suggests that change of sedimentation coefficient is not due to alteration of molecular weight of GCR binding subunit.
