Non-association between the polymorphism of collagen type Ⅰ alpha 1 gene and bone mineral density of postmenopausal women in Shanghai
- VernacularTitle:上海地区绝经后妇女Ⅰ型胶原?1基因多态性与骨密度无关
- Author:
Jianmin LIU
;
Hanmin ZHU
;
Huaidong SONG
- Publication Type:Journal Article
- Keywords:
Osteoporosis, postmenopausal;
Polymorphism (Genetics);
Bone density;
Collagen type Ⅰ alpha 1 gene
- From:
Chinese Journal of Endocrinology and Metabolism
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the polymorphisms of collagen type Ⅰ alpha 1 (COLIA1) gene and bone mineral density of postmenopausal women in Shanghai. Methods The polymorphisms of Sp1 binding site in COLIA1 gene were observed in 205 postmenopausal women by PCR, digested with MscⅠ restriction endonuclease and separated by 12% polyacrylamide gel electrophoresis. The capital letter S represents for the absence of the restriction endonuclease site, while the small letter s for the presence of the restriction endonuclease site. Bone mineral density at lumbar spine and femoral neck, etc. were measured by DEXA. Results Neither Ss nor ss genotypes of COLIA1 gene was found in our sample, all were SS genotype. Conclusion The contribution of Sp1 site variation of COLIA1 gene to bone mass might have a strong ethnic difference. The restriction fragment length polymorphisms of COLIA1 gene do not show relationship with the bone mass of postmenopausal women in Shanghai. The significance of this genetic marker of osteoporosis needs to be further explored in larger samples and in different areas.
