Bone formation by bone marrow stromal cell using tissue engineering methods in vitro and in vivo
- VernacularTitle:骨髓基质细胞体内外成骨的实验研究
- Author:
Dan JIN
;
Guoxian PEI
;
Qian WANG
- Publication Type:Journal Article
- Keywords:
Bone marrow;
Osteogenic;
Stromal cell;
Cell culture
- From:
Chinese Journal of Microsurgery
2000;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the osteogenic capability of bone marrow stromal cell (BMSc) using tissue engineering methods Methods The osteogenic potential in vitro of cultured BMSc in a conditional medium were examined by phase contrast microscopy,histochemistry stains technique The BMSc were cultured in combination with bioactive glass ceramic (BGC) materials Then the composite were implanted into the skeletal muscle beds in rabbits All implants were exmined by gross observation and histological examination Results The BMSc showed a similar property to those of osteoblasts and could synthesized mineralized new bone tissue in vitro New bone tissue can be observed in the composites of the BMSc and BGC materials Conclusions New bone tissue can be formed by tissue engineering methods
