Inhibition of protein phosphatases induces apoptosis in neuroblastoma cells
- VernacularTitle:抑制磷酸酶和tau过度磷酸化诱导神经细胞凋亡
- Author:
Ruohan XIA
;
Shengyuan LIU
;
Jianzhi WANG
- Publication Type:Journal Article
- Keywords:
Alzheimer disease;
Tau proteins;
Phosphorylation;
Neurons;
Apoptosis
- From:
Chinese Journal of Neurology
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between protein phosphatase inhibition, tau hyperphosphorylation and neuronal death seen in Alzheimer disease (AD). Methods Co culture of protein phosphatase inhibitor okadaic acid (OA) and neuroblastoma cells (SH SY5Y), by agarose gel electrophoresis to detect DNA fragmentation, and in situ hybridization by TdT mediated biotin labeled dNTP nick end labeling (TUNEL) to further detect the cell apoptosis. Results Incubation of SY5Y cells with 10 nmol/L OA for 24 or 48 hours led to the appearance of DNA fragmentation and a remarkable increase of positive cells from 2 16%?0 94% to 18 05%?3 57% ( P
