The soluble receptor flt-1_(n3) of vascular endothelial growth factor inhibits bladder carcinoma angiogenesis and growth
- VernacularTitle:血管内皮生长因子可溶性受体flt-1_(n3)对裸鼠人膀胱癌生长的影响
- Author:
Zhichao ZHANG
;
Zhiqing ZHANG
;
Gefei ZENG
- Publication Type:Journal Article
- Keywords:
Bladder neoplasms;
Carcinoma;
VEGF receptor
- From:
Chinese Journal of Urology
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To verify whether the extracellular domain of flt 1 has anti angionesis activity in vivo. Methods A recombinant plasmid pcDNA 3.1/flt 1 n3 was transfected into the human bladder carcinoma EJ cell by lipofectamin, which was constructed by inserting the N terminal first three IgG like domain of VEGF receptor flt 1 (flt 1 n3 ) into eukaryotic expression vector pcDNA 3.1. Results The rflt 1 was functionally expressed in stably transfected EJ and the product secreted in the medium could be specifically bind to rhVEGF165.The result showed that the bladder cancer transfected with rflt 1 had a lower microvessle density than the control. Conclusions It is proved that the expressed rflt 1 n3 can inhibit tumor growth and angiogenesis in nude mice model.
