Clone of apoptosis-related genes in prostate cancer cell by means of an improved subtractive hybridization
- VernacularTitle:改良消减杂交方法克隆前列腺癌细胞凋亡相关基因
- Author:
Chen SHAO
;
Feng ZHU
;
Wei YAN
- Publication Type:Journal Article
- Keywords:
Prostatic neoplasms;
Carcinoma;
Cell apoptosis;
Cenes
- From:
Chinese Journal of Urology
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up an apoptosis model of prostate cancer cell line and to clone and study the apoptosis related genes. Methods An apoptosis model of prostate cancer cell line DU-145 has been set up through induction by all transretinoic acid (ATRA).During the process of cell apoptosis the apoptosis-related gene was cloned by means of improved PCR-based subtractive hybridization from the apoptosis prostate cancer cell line DU-145 prostate cancer cells. Results During the process of DU-145 cell apoptosis,c-erb B-2 expression,TNF genes and some unknown apoptosis-related gene were observed.This has been accepted by Genebank,the accession number being AF174394. Conclusions ATRA-induced apoptosis of DU-145 cells is a complex process with multiple genes involved,some of which being unknown yet.
