The effects of ET antisence oligoneuleotide and eNOS genetic transfection on the intimal hyperplasia of venouse autografts
- VernacularTitle:联合转染eNOS基因反义ET核酸对自体移植静脉内膜增生的影响
- Author:
Chunxi WANG
;
Ziquan DUAN
;
Zhiqiang HUANG
;
Faqi LIANG
;
Qingbi SONG
- Publication Type:Journal Article
- Keywords:
Endothelin;
Nitric oxide synthase;
Endangium;
Rats,inbred strains
- From:
Chinese Journal of Current Advances in General Surgery
1999;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of endothelin(ET) antisence oligoneuceotide and nitric oxide synthase (NOS)genetic transfection on the intimal hyperplasia of venous autografts.Methods: The external jugule veins were autografted into abdominal aorta arteries in 20 Wistar rats, which were divided evenly into experimental and control group at random. The transplanted veins of experimental group were immersed in the adenovirus-mediated eNOS gene solution for 15 minutes just before anastomosis and coated with ET antisence olioneucleotide glue gel just after anastomosis, while the control ones were only immersed the physiologic saline for 15 minutes but nothing to be coated. The transplanted vascular samples were taken at 2 weeks after operation. The intimal thinkness (IH), degree of restenosis(DR), expression of PCNA, ETmRNA, NOS mRNA were determined by histology, transcription polymerase chain reaction.Results: The IH, DR and the expressions of ETmRNA were decreased while the expressions of eNOS mRNA was increased contrary to control group.Conclusion: Transfection of ET antisence oligoneuleotide and NOS gene can inhibit the intimal hyperlisia of venouse autogrfts,it is a prospective and idea genetic prophylactic therapy to intimal hyperplasia.
