Construction of monoclone cell lines of SP2/0-S 2S and detection of their expression
- VernacularTitle:乙型肝炎病毒表面抗原M蛋白真核表达细胞株的建立及其表达产物的检测
- Author:
Fang HE
;
Shan QIN
;
Liansan ZHAO
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus M-HBsAg Monoclone cell
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct a monoclone cell line expressing M-HBsAg.Methods:Transfected SP2/0 with pRc/CMV-S 2S by Calcium Phosphate Method,selected the cells by G418,detected HBsAg by Dot-blot、ELISA and Western-blot hybridization.Results:By selection of G418,G418 resistent cells were obtained;tests of Western-blot hybridization and ELISA demonstrated their expression of aimed protein.Conclusion:Monoclone Cell Lines of SP2/0-S 2S were obtained.
