Antitumor effect of human MCP－1 on human osteosarcoma bearing nude mice
- VernacularTitle:重组人单核细胞趋化因子-1对荷骨肉瘤裸鼠的治疗作用
- Author:
Zongxiong CHEN
;
Juliang BAO
;
Shuying ZHANG
- Publication Type:Journal Article
- Keywords:
Monocyte chemoattractant protein－1;
Osteosarcoma;
Mice,nude
- From:
Chinese Journal of Orthopaedics
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the inhibition effect of recombinant human monocyte chemoattractant protein－ 1(MCP－ 1) on implantation and growth of human osteosarcoma cells. Methods The method of protein fusion was used for the expression of MCP－ 1 in b.coli and then the MCP－ 1 was extracted and purified. Fifty nude mice were divided into 10 groups. For A1－ 4 groups, the 5 mice in each group were locally injected with doses of 1? g, 10? g, 100? g, 1 mg of MCP－ 1 at the same time when 4.4? 106 osteosarcoma cells were implanted in vivo. For B0－ 4 groups, 5 mice in each group, the injection were given 2 weeks later when there was the formation of the tumor mass at doses of 0? g(0.2 ml normal saline), 1? g, 10? g, 100? g, 1 mg of MCP－ 1 every other day. Five mice in group C were injected with dose of 0.2 ml NS as control. Results Implantation of osteosarcoma cells were completely prevented among mice of group A2－ 4, tumor inhibiting effect even in group A1 and the rate of tumor inhibition was 69.69％ . AKP values in mice of group A1－ 4 were much lower than those of group B0(P
