Effects of interferon-? on thyroglobulin and thyroid peroxidase gene expressions in FRTL5 cells induced by thyroid stimulating antibody
- VernacularTitle:?干扰素对甲状腺刺激抗体作用下FRTL5细胞TG、TPO基因表达的影响
- Author:
Zhenfang YUAN
;
Yan GAO
;
Changhong LI
- Publication Type:Journal Article
- Keywords:
Interferon ?;
Gene expression;
Thyroglobulin;
Thyroid peroxidase;
Thyroid stimulating antibody
- From:
Chinese Journal of Endocrinology and Metabolism
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of interferon ? (IFN?) on thyroglobulin (TG) and thyroid peroxidase (TPO) gene expressions in FRTL5 cells induced by thyroid stimulating antibody (TSAb). Methods TSAb crude fraction was extracted by polyethylene glycol 4000. Recombinant rat interferon ? (0, 1, 10, 10 2, 10 3 U/ml) was added to the FRTL5 cells induced by TSAb, and then expressions of TG, TPO mRNA were measured by Northern blot, the cell growth was measured by 〔 3H〕 thymidine incorporation. Results (1)TSAb increased TG, TPO gene expressions, and 〔 3H〕 thymidine incorporation. (2)Interferon ?inhibitedTSAb inducedTG, TPOgeneexpressionsand〔 3H〕 thymidine incorporation. Conclusion Interferon ? inhibits the growth and function of thyrocytes induced by TSAb, which suggests that interferon ? might regulate thyroid function in Graves' disease.
