An experimental study on the effect of extract of leave ginkgo biloba(EGb24/6)on protection of the neurons and the observation of ultra-structure following nerve injury of sciatic nerve in rats
- VernacularTitle:银杏叶提取物EGb_(24/6)对受损神经神经元的保护作用及其对神经元超微结构的影响
- Author:
Feng ZHANG
;
Yudong GU
;
Jianguang XU
- Publication Type:Journal Article
- Keywords:
Plant extracts;
Sciatic nerve;
Wounds and injuries； Neurons;
Apoptosis
- From:
Chinese Journal of Orthopaedics
1999;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of extract of leave ginkgo biloba (EGb) for protection of the motor and sensory neurons axotomy-induced in rats. Methods Thirty male Sprague-Dawley rats weighing 180－ 220 g (8－ 9 weeks of age), were randomly divided into control group and EGb24/6 group. Models of sciatic nerve injury were established by transection of left sciatic nerve at 0.5 cm away from the points of muscular innervation. The proximal stump of sciatic nerve was double ligated to inhibit nerve regeneration. The distal end of the nerve was implanted into the biceps femoris. Then 2 ml of normal saline was given to control group, and 2 ml of EGb24/6 to the EGb24/6 groups daily. The rats were sacrificed in 7,14,28 days after surgery respectively, and then 4％ paraformaladehyde was perfused transcardially. The L4－ 6 spinal cord and L5 dorsal root ganglion (DRG) were harvested. HE staining and CV technique were used for detection of the motor neurons and compared with contra-lateral side. TUNEL technique was used for detection of apoptotic motor and sensory neurons. Eletron microscopic technique was used for observation of change of L5 DRG neurons. Result The number of motor neurons was significantly higher in EGb24/6 group in each postoperative period than that in control group (P
