DNA polymorphism of Prevotella intermedia in subgingival plaque in the patients with periodontitis
- VernacularTitle:中间普里沃菌基因多态性及其在成人牙周炎患者龈下菌斑中的分布
- Author:
Jincai ZHANG
;
Bo WU
;
Ping HUANG
- Publication Type:Journal Article
- Keywords:
Prevotella intermedia;
Genotype;
Periodontal disease
- From:
Journal of Practical Stomatology
1996;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
砄bjective:To analyze the DNA polymorphism of Prevotella intermedia(P.i ) and their distribution at diseased and healthy sites in adult patients with periodontitis.Methods:101 P.i strains were obtained from subgingival plaque of the adults with periodontitis.Aabitrarily primed polymerase chain reaction (APPCR)with OPA 03 and OPA 13 as primers was used to study the genetype of P.i .Results:The OPA 03 primer AGTCAGCCAC grouped 101 P.i strains into 7 different genotypes,while OPA 13 CAGCACCCAC divided 97 P.i strains into 12 genotypes.There was mainly one genotype of P.i in each subject,and mainly one genotype of P.i in individual diseased and healthy site.Also,several different genotypes of P.i were found in a identical subject and at a identical site.The predominant genotypes of P.i in diseased sites were mostly the same to that in healthy sites,no special genotype of P.i which was associated to periodontal status was found.Conclusion:Overgrowth of P.i without special genetype may be a pathogen for the pathogenesis of periodontitis.
