Arg-Gly-Asp peptide on pure golden coated titanium regulates osteocalcin mRNA expression in osteoblasts in vitro
- VernacularTitle:生物材料表面固定粘附肽调节小鼠成骨细胞骨钙素的表达
- Author:
Hui HUANG
;
Xuanxiang MA
;
Tao FU
- Publication Type:Journal Article
- Keywords:
Adhesive peptide;
Osteocalcin;
RNA blotting
- From:
Journal of Practical Stomatology
1996;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
砄bjective:To determine the effect of Arg Gly Asp(RGD) containing peptide on the expression of osteocalcin mRNA expression in mouse calvarial osteoblasts.Method:Titanium samples were coated with pure golden and then immersed into Arg Gly Asp Cys (RGDC) solution (2 mmol/L) for overnight.The materials coated with Arg Gly Asp Cys (RDGC) or without treatment were used as controls.Mouse calvarial osteoblasts were isolated and seeded on the surfaces and harvested in 10,15,and 21 d respectively.Osteocalcin mRNA was detected by Northernblot hybridization.Results:Osteocalcin expresion was found in the cells on RGDC coated surface on day 15,while that was found in the cells in other groups at same level on day 21.Conclusion: RGD peptide can promote the osteocalcin expression in mouse osteoblasts.
