Molecular design for antagonisting C5a anaphylatoxin and the activity examination of the peptide
- VernacularTitle:人C5a过敏毒素拮抗剂的分子设计及其活性检测
- Author:
Fenglin LV
;
Xihua ZHU
;
Ping ZHENG
- Publication Type:Journal Article
- Keywords:
Molecular design C5a anaphylatoxin C5a receptor(CD88)
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To discover some high hydrophilic profiles of the human C5a anaphylatoxin based on relationship between the structure and function of the protein and the protein molecular design principles.Methods:The peptides were synthesized by 431A automatic peptide synthesizer,purified by PHLC and confirmed by caplilliary electrophoresis.Results:The N terminus No.9 30 profile of the C5aR(P22) could interacte with anti C5aR McAb(S5/1,from Serotic Co.),as determined by ELISA.Furthermore,it could be inhibited OD490 values remarkably by 10.0 ?g/L rhC5a(P
