Pingyangmycin(bleomycin A5)-mediated electrochemotherapy on human prostate cancer cell line PC-3M in vitro
- VernacularTitle:电脉冲化疗对人前列腺癌细胞系PC-3M的体外作用
- Author:
Xihua CHANG
;
Jinguo WANG
;
Yong XIA
- Publication Type:Journal Article
- Keywords:
Prostatic neoplasms;
Carcinoma;
Electrotherap
- From:
Chinese Journal of Urology
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the efficacy of electrochemotherapy with pingyangmycin (bleomycin A5) for the treatment of human prostate cell line PC 3M in vitro. Methods The prostate cancer cells were treated by pingyangmycin alone, electric pulse alone and by electrochemotherapy, i.e. electric pulses were delivered after pingyangmycin was given. Cytotoxicity was examined by MTT assay and histological examination. Results The maximum nontoxic dose for pingyangmycin was 0.1 ?g/ml; The maximum voltage not showing cytotoxicity was 125 V/mm.The 50% inhibitory concentration was 100 times reduced in electrochemotherapy than pingyangmycin alone. Conclusions The cytotoxicity of pingyangmycin can be enhanced by electric impulses. Pingyangmycin may be a novel drug for electrochemotherapy.
