Dexamethasone on apoptosis and expression of Fas mRNA in adult rats with permanent middle cerebral artery occlusion
- VernacularTitle:氟美松对成年大鼠持续性局灶脑缺血后细胞凋亡和Fas基因表达的作用
- Author:
Tao FENG
;
Ke ZHU
;
Xiaokun QI
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia;
Dexamethasone;
Apoptosis;
Gene expression
- From:
Chinese Journal of Neurology
1999;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible role and mechanism of the neurotoxic effect of dexamethasone on adult rats after having focal cerebral ischemia. Methods The rat model of focal cerebral ischemia was established by permanent middle cerebral artery occlusion (MCAO) One hour after ishemia,the experimental groups were treated with dexamethasone (5 mg/kg) while the control groups were treated with saline TUNEL staining and In-suit RT-PCR were used to show the changes of apoptosis and the expression of Fas mRNA at ipsilateral cerebral hemisphere. Results TUNEL positive cells were present in a time from 48 h to 72 h, localizing at peripheral ischemic area The expression of Fas mRNA at peripheral ischemic area in control groups began at 12 h, peaked at 24 h, and decreased to a lower level at 48 h and 72 h, and returned to the baseline at 120 h Treatment with dexamethasone after ischemia made apoptosis present at 24 h and the number of TUNEL positive cells at 48 h exceeded that in the control group at 48 h ( P
