The Expression of P53 and Phosphorylation of H2AX in Germ Cells of Varicocele Rats.
10.4111/kju.2006.47.12.1354
- Author:
Won Jin CHO
1
;
Moon Hwan YEO
;
Ho Jin YOU
;
In Youb CHANG
;
Hyung Yoon MOON
;
Chul Sung KIM
Author Information
1. Department of Urology, College of Medicine, Chosun University, Gwangju, Korea. mu-hn@hanmail.net
- Publication Type:Original Article
- Keywords:
DNA damage;
Varicocele;
P53;
gamma-H2AX
- MeSH:
Adult;
Animals;
Blotting, Western;
DNA Damage;
Germ Cells*;
Humans;
Immunohistochemistry;
Male;
Orchiectomy;
Phosphorylation*;
Rats*;
Rats, Sprague-Dawley;
Spermatogenesis;
Spermatozoa;
Testis;
Varicocele*
- From:Korean Journal of Urology
2006;47(12):1354-1360
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To explore the expressions of P53 and phosphorylation-H2AX in varicocele-induced rat testes. MATERIALS AND METHODS: A total of 16 adult male Sprague-Dawley rats underwent an operation; 12 underwent an experimental varicocele and 4, as controls, were sham-operated. Groups of 4 varicocele-induced rats underwent a left orchiectomy after 2 or 3 weeks, or both orchiectomies after 4 weeks. The sham-operated rats underwent both orchiectomies after 4 weeks. Sections of both testes from each animal were studied. The changes in the expressions of P53 and phosphorylation of H2AX were determined using immunohistochemistry and western blot. RESULTS: Immunohistochemical staining of the left testes in the varicocele- induced rats showed that the expressions of P53 and phosphorylation of H2AX had not begun 2 weeks postoperatively, but remarkable results were observed after 3 and 4 weeks. Both testes of the varicocele-induced rats showed the expressions of P53 and phosphorylation of H2AX after 4 weeks, with the left testes being more distinctive in immunohistochemical staining compared to the right. Western blot of the left testes in the varicocele- induced rats also showed unclear expressions of P53 and gamma-H2AX after 2 weeks. Considerable distinction was seen after 3 and 4 weeks compared to the control group. CONCLUSIONS: Our results suggest that experimental varicocele is associated with increased sperm DNA damage. These changes may be related to abnormal spermatogenesis.
