Effects of estrogen on the cultured osteoclasts in vitro
- VernacularTitle:雌激素对体外培养破骨细胞功能的影响
- Author:
Daohai LU
;
Zhongmin ZHANG
;
Yinghong CHEN
- Publication Type:Journal Article
- Keywords:
Estrogens;
Osteoclast;
Bone resorptionx
- From:
Chinese Journal of Geriatrics
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of estrogen on the cultured osteoclasts in vitro. Methods The acid phosphatase (ACP) and tartrate resistant acid phosphatase (TRAP) activities were measured kinetically and the introcellular hydrogen ions were calculated with the confocal laser scanning microscopy(CLSM). The area and number of the resorption pits were determined with the Leica Quantimet 500 system. Bone resorption pits were observed with scanning electronicmicroscope. Results With the estrogen concentration increased, the ACP and TRAP activities decreased from (1 69?0 13) U/L and (1 60?0 14) U/L to (1 16?0 31) U/L and (0 93?0 34) U/L, respectively (experiment group vs control group, P
