PDGF-B and BMP-7 mRNA expression in the interface membrane around aseptically loosened prosthesis
- VernacularTitle:无菌性松动假体-骨界面溶骨因子和成骨因子表达的对比研究
- Author:
Xu CAI
;
Jifang WANG
;
Shibi LU
- Publication Type:Journal Article
- Keywords:
Joint prosthesis;
Platelet-derived growt factor;
Bone morphogenetic proteins;
Prosthesis failur
- From:
Chinese Journal of Orthopaedics
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the expression of osteolysis and osteogenesis cytokines in the interface membrane around aseptically loosened prosthesis for the elucidation of the biological mechanism of osteolysis caused by wear particles at bone-implant interface. Methods Reverse transcription polymerase chain reaction(RT-PCR) and computerized image processing were adopted to determine the mRNA expression of PDGF-B and BMP-7 respectively in the interface membrane(IM) at osteolysis region(with wear particles) and non-osteolysis region. Normal synovial membrane(NSM), OA synovial membrane(OASM) and normal cancellous bone(NCB) were obtained as negative or positive control. Results Expression of PDGF-B mRNA from high to low was OASM, IM at osteolysis region, NCB, IM at non-osteolysis region and NSM(P
